A dilution in chemistry reduces the concentration of a substance in a solution.A serial dilution is the repeated dilution of a solution.Concentration curves on a logarithmic scale or when you are determining the density ofbacteria are some of the experiments it is commonly performed in.In experimental sciences, serial dilutions are often used.
Step 1: The proper dilution liquid should be determined.
It's very important that the liquid that you will be using is of good quality.Many solutions will be reduced in distilled water, but this isn't always the case.You will want to use culture media if you are diluting cells.Every serial dilution will use the liquid you choose.If you are unsure what liquid to use, ask for help or check online to see if other people have done the same thing.
Step 2: Test tubes with 9 liters of liquid.
These tubes are used as dilution blanks.You will be adding your sample to the first tube and then diluting it into the following tubes.It is important to label your tubes before you begin so you don't get confused.The 10-fold dilution starts from the undiluted tube.The first tube will be a 1:10 dilution, the second a 1: 100, and the third a 1000.You don't want to waste tubes or diluting liquid if you know the number of dilutions you need to do.
Step 3: Prepare a test tube with your solution.
The minimum amount is 1 mL of solution.You won't have any remaining solution if you only have 1 mL.The tube US should be labeled for a solution.Before starting any dilutions, mix your solution thoroughly.
Step 4: The first dilution should be done.
Transfer the undiluted solution from the US to the test tube labeled 1:10 containing 9 mL of the dilution liquid with a pipette.There is a small amount of the solution in the liquid.The solution has been reduced by 10.
Step 5: The second dilution should be performed.
If you want to do the second serial dilution, you need to take 1 mL of solution from tube 1:10 and put it in the tube 1: 100.Add tube 1:10 to the next tube.After dilution, mix the tube 1: 100 again.The solution from the test tube has been changed into a different one.
Step 6: This procedure can be extended to perform longer serial dilutions.
As necessary, this process may be repeated many times.In an experiment involving concentration curves, you can use a serial dilution to create a series of solutions.
Step 7: The final dilution ratio is calculated using a serial dilution.
The total dilution ratio can be determined using the dilution factor of each step.The equation Dt is used to show the total dilution factor and the dilution ratio.Let's say you did a 1:10 dilution of your liquid 4 times.The final dilution factor of the fourth tube in your serial dilution is 1:10,000.The concentration of your substance has been reduced by 10,000 times.
Step 8: The concentration of the solution should be determined after the dilution.
You will need to know your starting concentration to determine the final concentration of your solution.The formula for the equation is Cinitial/D, where C final is the end concentration of the solution and C initial is its starting concentration.What is the final concentration of your sample if you started with a solution of 100,000 cells per mL and a dilution ratio of 1,000?The number of cells per mL is calculated using the equation.
Step 9: All units match, confirm that.
You want to make sure that your units match at the end of the calculation.Make sure you end with the same number of cells as you started.Your final concentration must be part of your starting concentration.