- Prepare 800 mL of distilled water in a suitable container.
- Add 11.7 g of EDTA to the solution.
- Slowly adjust the pH to 8.0 with 2 M NaOH. (Unless using the salt, EDTA often requires extended time and basic pH conditions to completely dissolve.) Store for up to 3 mo at 4°C.
How do you make a 2% EDTA solution?
- Stir 186.1 g disodium ethylenediaminetetraacetate•2H2O into 800 ml of distilled water.
- Stir the solution vigorously using a magnetic stirrer.
- Add NaOH to adjust the pH to 8.0. ...
- Dilute the solution to 1 L with distilled water.
- Filter the solution through a 0.5-micron filter.
How do you make a standard EDTA solution?
EDTA Solution Preparation Add 0.05 g magnesium chloride hexahydrate, three pellets of NaOH and add about 200 mL of distilled water to dissolve. The EDTA will dissolve slowly over a period of a half an hour.
What is EDTA and its uses?
A chemical that binds certain metal ions, such as calcium, magnesium, lead, and iron. It is used in medicine to prevent blood samples from clotting and to remove calcium and lead from the body. It is also used to keep bacteria from forming a biofilm (thin layer stuck to a surface). It is a type of chelating agent.
What does EDTA do in dentistry?
EDTA works by reacting with the mineral components of the hard tissues of the tooth. The chelating agent EDTA softens the tissues, decalcifying the canal walls. It facilitates the mechanical preparation of the root canal and further removal of the smear layer, which opens dentin tubules.