How do you read a GC mass spec?

How do you read a GC mass spec?

- The X-Axis: Retention Time. Usually, the x-axis of the gas chromatogram shows the amount of time taken for the analytes to pass through the column and reach the mass spectrometer detector. - The Y-Axis: Concentration or Intensity Counts. - Differences in Gas Chromatogram Models.

How does mass selective detector work?

- The MS will break each separated compound coming from the GC into ionised fragments. - The fragments then go through a process of acceleration and deflection whilst traveling through a short tunnel and being exposed to a magnetic field.

What does GC mass spec stand for?

Gas chromatography–mass spectrometry

What is the difference between mass spectrometry and gas chromatography?

The key difference between gas chromatography and mass spectrometry is that gas chromatography is important in separating components in a mixture, whereas mass spectrometry is useful in calculating the exact molecular weight of the sample components.

What is the purpose of mass spectrometry?

Mass spectrometry is an analytical tool useful for measuring the mass-to-charge ratio (m/z) of one or more molecules present in a sample. These measurements can often be used to calculate the exact molecular weight of the sample components as well.

How does GC mass spectrometry work?

The GC works on the principle that a mixture will separate into individual substances when heated. The heated gases are carried through a column with an inert gas (such as helium). As the separated substances emerge from the column opening, they flow into the MS.

How do you read a GC spectrum?

https://www.youtube.com/watch?v=9j65SIlv4hM

What data do you get from a GC mass spec?

It records the total number of ions entering the mass analyzer from the column. The chromatogram produced is called the total ion chromatogram. Each point in the chromatogram is a mass spectrum.

Why is gas chromatography used with mass spectrometry?

Analyzing small and volatile molecules When combined with the detection power of mass spectrometry (MS), GC-MS can be used to separate complex mixtures, quantify analytes, identify unknown peaks and determine trace levels of contamination.

What does a GC measure?

A gas chromatograph (GC) is an analytical instrument that measures the content of various components in a sample. The analysis performed by a gas chromatograph is called gas chromatography.