In‑Fusion Cloning is a highly efficient, ligation-independent cloning method, based on the annealing of complementary ends of a cloning insert and linearized cloning vector. This technology ensures easy, single-step directional cloning of any gene of interest into any vector at any locus.
What type of cloning is infusion?
Infusion cloning is a simple one step cloning technique where the gene of interest is annealed into a vector based on complementary flanking sequences.
What is fusion PCR?
Overlap extension or fusion PCR is thought to be a simple and easy method to produce fusion DNA fragments without the need for restriction enzyme digestion and DNA ligation. We describe how these overlap sequences can be used for fusion DNA construction, in-frame gene fusion, and cloning in yeast.
What are the benefits of In Fusion cloning over traditional cloning approaches?
The efficiency of In-Fusion Cloning kits is over 95%, and just one quick reaction allows you to recover your final construct. This versatile system easily outpaces any traditional cloning method, including ligation and TA cloning.
What are the types of cloning?
- Gene cloning, which creates copies of genes or segments of DNA.
- Reproductive cloning, which creates copies of whole animals.
- Therapeutic cloning, which creates embryonic stem cells.
What does scarless cloning mean?
Return to Cloning & Synthetic Biology. The group of cloning methods we refer to as "seamless cloning" typically combine attributes of more established cloning methods to create a unique solution to allow sequence-independent and scarless insertion of one or more fragments of DNA into a plasmid vector.
What are the types of cloning vectors?
The different types of vectors available for cloning are plasmids, bacteriophages, bacterial artificial chromosomes (BACs), yeast artificial chromosomes (YACs) and mammalian artificial chromosomes (MACs).