Spectrophotometry is a standard and inexpensive technique to measure light absorption or the amount of chemicals in a solution. It uses a light beam which passes through the sample, and each compound in the solution absorbs or transmits light over a certain wavelength.
What two things can a spectrophotometer be used for?
A spectrophotometer is a device used to measure light at a specific wavelength. It consists of two parts: a spectrometer and a photometer.
What can you analyze with a spectrophotometer?
The spectrometer, or spectrophotometer, is an analytical instrument used to identify or confirm the chemical species, chemical structure, or concentration of substances in a sample. The instrument emits an energy source to pass through a solution and measure light intensity at different wavelengths.
What are the uses of spectrophotometer?
Spectrophotometers are widely used in various disciplines such as physics, molecular biology, chemistry and biochemistry. Applications for specs include measurement of substance concentration such as protein, DNA or RNA, growth of bacterial cells, and enzymatic reactions.
What is an example of spectrophotometry?
An example of an experiment in which spectrophotometry is used is the determination of the equilibrium constant of a solution. The amount of light that passes through the solution is indicative of the concentration of certain chemicals that do not allow light to pass through.
What experiments would a spectrophotometer be useful?
The spectrophotometer can be used in a variety of spectroscopy experiments including determining the peak wavelength to collect data on solution concentration for studies of Beer's law or to monitor rates of reaction; collecting a full wavelength spectrum to measure absorbance, percent transmittance, fluorescence, or
How does UV spectrophotometer measure absorbance?
Absorbance is measured using a spectrophotometer or microplate reader, which is an instrument that shines light of a specified wavelength through a sample and measures the amount of light that the sample absorbs.
How does a spectrophotometer measure absorbance of a Coloured solution?
The spectrophotometer measures the absorbance of each different wavelength by adjusting the monochromator. Depending on the color of the solution, the absorbance will peak at specific wavelengths.