Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
Is Cy3 red or green?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).
Cy5. 5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments. This reagent can replace NHS esters of Cy5.
What Colour is Cy3?
Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
What does Cy3 stain?
In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids. The Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore.
What Cy3 and Cy5 denotes in microarray technology?
The microarray is analyzed in a scanner, which uses laser light to cause each spot to fluoresce and measures the relative intensity of emission, typically at green (Cy3) and red (Cy5) wavelengths.
What is Cy5 used for?
Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
What is the wavelength for Cy3?
Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets.
What wavelength is TRITC?
532 nm
Is cy2 green?
Cyanine, Cy™2 Technical Information Cy™2 - conjugates have maximum absorption/excitation at 492 nm and fluoresce with a peak around 510 nm in the green region of the visible spectrum like FITC conjugates (520 nm), but they are more photostable and less sensitive to pH changes than FITC.
Are PerCP and PerCP Cy5 5 the same?
PerCP-Cy5. 5 (Ex-Max 482 nm/Em-Max 695 nm): is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5. 5 is not subject to photobleaching like PerCP and can be used with stream-in-air flow cytometers.
What is a Cy5 label?
Cy5/ Cyanine 5 are popular fluorescent dyes compatible with various detection techniques and standard filters. This class includes far-red fluorescence-emitting dyes with excitation/emission maximum 646/662 nm.