What factors should you consider when trying to choose an ELISA test?
What factors should you consider when trying to choose an ELISA test?
- The species for testing.
- The type of analyte for detection.
- Type of antibodies.
- Linearity and recovery experiments.
- The system used for detection range.
What is the most common ELISA test?
Sandwich ELISAs are the most common type of ELISA. Two specific antibodies are used to sandwich the antigen, commonly referred to as matched antibody pairs. Capture antibody is coated on a microplate, sample is added, and the protein of interest binds and is immobilized on the plate.
How many samples can be tested in an ELISA kit?
It is recommended to test standards and samples at least in duplicate. How many samples can be analyzed with one 5-plate ELISA kit? When analyzing all samples in duplicate, you can run in total 200 samples (one dilution per sample, and including 7 standard dilutions and one blank for each plate).
Test kits cost from $1.20 per test for ELISA to more than $30 for western blot.
How long are ELISA kits good for?
6 months
What is an ELISA test kit?
ELISA kits are ready-to-use immunoassay kits that contain all reagents required to perform the ELISA (Enzyme-Linked Immunosorbent Assay). Antibodies linked with enzymes are used to quantify the results. Various formats (see below) exist and the antibody antigen interaction principle is different for each format.
What does an ELISA kit contain?
These ELISA kits include pre-coated plates with capture/detection antibodies, standards, buffers and accessory reagents. Each kit goes through fit-for-purpose validation and stability testing to ensure precision, accuracy, sensitivity, specificity, and reproducibility.
How does an ELISA kit work?
An antigen ELISA can tell whether an animal is infected with a virus by detecting it directly. For an antibody ELISA, antigens are stuck onto a plastic surface, a sample is added and any antibodies for the disease we are testing for will bind to the antigens.
What can ELISA be used for?
The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some examples include: diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum.
How long do ELISA plates last?
Ready-to-use ELISA kits from good suppliers may be stored for two years at 4°C without any problem. With home-made ELISA kits it is a completely different story. For any new measurement one has to coat a new plate, because after storage of some days the plates don't perform as well as before.
How do you store ELISA kits?
How should I store U-CyTech's ELISA products upon arrival? Store the kits and antibody pairs at 4 °C upon arrival. We recommend storing the lyophilized SPP conjugate vial at -20 °C in the dark to maintain long-term stability.