VWR®'s Blue Light Transilluminator provides a safe and cost effective alternative to UV transilluminators for viewing gels stained with dyes excited in the blue light spectrum. Blue light excitation does not damage DNA samples and these dyes can be safe alternatives to hazardous Ethidium Bromide.
What is a transilluminator light?
A blue light transilluminator is a 'safe' light source, in that the user is not exposed to harmful UV radiation and samples are free from photo-nicking. For researchers working with nucleic acid (DNA/RNA) or protein gels (Coomassie, silver stain) gels, these systems offer a great value and price.
What is a Transilluminator used for?
An ultra-violetultra-violetLow-pressure UV lamps offer high efficiencies (approx. 35% UV-C) but lower power, typically 1 W/cm power density (power per unit of arc length). Amalgam UV lamps utilize an amalgam to control mercury pressure to allow operation at a somewhat higher temperature and power density.https://en.wikipedia.org › Ultraviolet_germicidal_irradiationUltraviolet germicidal irradiation - Wikipedia (UV) transilluminator is a standard piece of equipment used in life science laboratories for visualization of target DNAs and proteins. The UV transilluminator works by emitting high levels of UV radiation through the viewing surface.
What is the UV illuminator used for?
UV transilluminators are used in molecular biology laboratories to view DNA or RNA that has been separated by electrophoresis through an agarose gel. Exposing the stained gel to a UV light source causes the DNA to fluoresce and become visible.
What is the UV box used for in gel electrophoresis?
Ultraviolet (UV) transilluminators or UV light boxes are used in biotechnology for visualization of nucleic acids (DNA or RNA) after gel electrophoresis and ethidium bromide staining.
Why do you need a UV Transilluminator to observe your DNA bands?
Introduction: UV Transilluminator This technique is used wherever the researcher needs to be able to view their sample, for example sizing a PCR product, purifying DNA segment after a restriction enzyme digest, quantifying DNA or verifying RNA integrity after extraction.
Why is UV light used to visualize DNA after electrophoresis?
Research laboratories commonly use fluorescent DNA stains because they are extremely sensitive, making it easy to quantify small amounts of DNA. In order to visualize the DNA fragments, an ultraviolet (UV) light source (such as a transilluminator) is used to excite the fluorescent molecules.