Scope. 1.1 This test method covers the measurement of the specific surface area of precipitated silicas exclusive of area contained in micropores too small to admit hexadecyltrimethylammonium bromide (cetyltrimethylammonium bromide, commonly referred to as CTAB) molecules.
Why is CTAB used?
CTAB (also called hexadecyltrimethylammonium bromide) is a cationic detergent that facilitates the separation of polysaccharides during purification while additives, such as polyvinylpyrrolidone, aid in inactivating polyphenols. CTAB based extraction buffers are widely used when purifying DNA from plant tissues.
How do I create a CTAB extraction buffer?
- 2 % CTAB.
- 100 mM Tris (pH 8.0)
- 20 mM EDTA.
- 1.4 M NaCl.
- 1-2 % PVP polyvinylpyrrolidone 40.
- 0.2 % Beta mercaptoethanol Add just before use; (20 µl per 10 ml solution)
What is Lung CTAB?
• CTAB no rrw = Lungs are “Clear to. auscultation bilaterally, no rales, rhonchi or. wheezes”
Is CTAB soluble in water?
Cetyltrimethylammonium Bromide is a cationic detergent suitable for the isolation of high molecular weight DNA in plants and other organisms. Solubility : Soluble in water (36.4 g/l at 20° C), methanol (>200 g/l at 20° C), alcohol (Very soluble), acetone (Slightly soluble), and chloroform.
What is the full name of CTAB?
Cetrimonium bromide (Cetyltrimethylammonium bromide or CTAB) is a surfactant and antiseptic agent with various antibacterial, antifungal and antiviral properties.