EcoRI is a restriction endonuclease enzyme, isolated from E. coli bacterium, which cuts through DNA at the palindromic sequence 5' GAATTC 3', and the opposite sequence on the other strand, 3' CTTAAG 5'. EcoRI 'cuts' through the sequence between the adenine base and the glycine base on each strand, leaving sticky ends.
Where is EcoRI found?
bacteria Escherichia coli
How many restriction sites are there for EcoRI?
Note, after a reaction with the EcoRI enzyme, that the DNA of species A is cleaved into three fragments, corresponding to two EcoRI restriction sites, whereas that of species B is cleaved into four fragments, corresponding to three EcoRI restriction sites.
Where does EcoRI cleave DNA?
GAATTC sites
Where does the EcoRI enzyme cut?
DNA
Where does EcoRI cleave?
The EcoRI endonuclease is a symmetric homodimercl 3, that cleaves the sequence GAATTC between G and A on both DNA strands(') (Fig. 1).
What is the EcoR1 cut site?
In molecular biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G↓AATTC, which has a palindromic, complementary sequence of CTTAA↓G.
How does EcoRI cleave?
How does EcoRI cleave its recognition site on DNA? The two protein subunits of the EcoRl restriction enzyme interact symmetrically with the recognition site on DNA, so that each subunit is in position to cleave one strand of the DNA.
What is the restriction site for EcoRI?
Cleavage of DNA at an EcoRI restriction site The restriction endonuclease enzyme EcoRI recognizes the ssDNA sequence 5'-GAATTC'-3, and introduces a single-strand cut between the G & A nucleotides. This recognition site is a palindrome: the opposite strand also reads 5'-GAATTC'-3 and will be cut in the same manner.
How many restriction sites are there?
History. Restriction enzymes recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to these sequences. Approximately 3,000 restriction enzymes, recognizing over 230 different DNA sequences, have been discovered.
How many fragments are produced by EcoRI?
When cut by EcoRI, the cDNA will yield 0.5, 1.5, and 2 kilobase fragments. Digesting with both EcoRI and HindIII, will yield 0.5, 1, and 1.5 kilobase fragments.
Where does restriction enzyme ECOR1 cut DNA?
EcoR1 cuts DNA at the recognition sequence between the G and A nucleotides (see legend for Fig. 1) to produce a 16-bp fragment that remains attached to the microparticle surface and a 74-bp fragment free to diffuse into the bulk solution.
How does EcoRI bind to DNA?
EcoRI is a type II restriction endonuclease with a palindromic target site on DNA—GAATTC—that it binds to as a dimer. Ostensibly, the sequence specificity in EcoRI develops from hydrogen bonds between the purines and protein, and contacts between protein, the pyrimidines and the DNA backbone.