S.O.C. Medium is supplied as 10 x 10 mL bottles of liquid medium with the following composition: 2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4, and 20 mM glucose.
What is sob used for?
SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells. SOC is used for initial growth of competent cells and the transformation procedure.
What is meant by SOC medium?
SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.
Can I use LB instead of SOC medium?
SOC is not mandatory, Instead you can also use LB broth. Transformation is depends on handling, heating-cooling and quantified DNA.Jul 1, 2018
Does SOC medium expire?
Which is better? SOC medium can be stored at either 4°C or Room Temperature depending on how fast it will be used. Storing at Room Temperature is convenient and adequate for short term usage (weeks to a couple of months). For long term storage, we recommend storing at 4°C.
How to make SOB media?
SOB medium is prepared without Mg++ by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 2 ml of 5M sodium chloride, and 2.5 ml of 1M KCl in water to a final volume of ≈ 1000 ml (980 ml).
How do I get SOC from SOB?
For SOC (=SOB+glucose): Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)Jul 8, 2021
Why is SOC medium used?
SOC Medium is a rich medium used primarily to aid recovery of bacterial competent cells following transformation. Use of SOC medium improves the molecular uptake whilst stabilizing the cells rapidly and so maximizing the efficiency of competent cells.
Is SOC better than LB?
SOC is an 'optimal medium' aimed at providing all nutrients and additives that may help cells recover after heat shock. Recovery is better with LB than plating the cells directly after heat shock.Jul 1, 2018