Red blood cell counts (RBC), mean corpuscular volume (MCV) of red blood cells, and platelet counts may be determined using an automated device such as a Coulter counter to take direct measurements.
What is Coulter method?
The Coulter Principle The Coulter method of sizing and counting particles is based on measurable changes in electrical impedance produced by nonconductive particles suspended in an electrolyte. A small opening (aperture) between electrodes is the sensing zone through which suspended particles pass.
What is the principle of cell counter?
The traditional method for counting cells is electrical impedance, also known as the Coulter Principle. Whole blood is passed between two electrodes through an aperture so narrow that only one cell can pass through at a time. The impedance changes as a cell passes through.
What is the principle of Coulter Counter method?
The Coulter counter uses the principle that the electrical resistance of a conducting liquid is increased by the addition of an insulating material. Particles are assessed individually. To obtain adequate sensitivity, the volume of liquid measured must be similar to the volume of the particle.
What is Beckman Coulter method?
The Coulter Principle is based on the detection and measurement of changes in electrical resistance produced by a particle or cell suspended in a conductive liquid (diluent) traversing through a small aperture. When particles or cells are suspended in a conductive liquid, they function as discrete insulators.
What is the purpose of a Coulter counter and flow cytometry?
The Coulter counter is a vital constituent of today's hospital laboratory. Its primary function is the quick and accurate analysis of complete blood counts (often referred to as CBC). The CBC is used to determine the number or proportion of white and red blood cells in the body.